Precursor/Product Isotope Matching

Precursor/Product Isotope Matching (PRISOMATCH) was invented by SiDMAP scientists as the disease and drug effect specific protein turnover biomarker tool for isotopolomics. PRISOMATCH requires no tracers but only naturally or medically introduced small variations in precursor isotope enrichment, followed by protein mass shift analyses as described in provisional claims (United States Patent Application 20090176199)

Specific proteins are captured using any of the commonly available protein isolation and antibody precipitation methods before mass shift analysis over time.

Single low enrichment 13C, Deuterium or 15N containing substrates are all applicable, and natural isotope variations also allow matching among proteins which are synthesized in disease at different rates.

PRISOMATCH is the primary tool for in vitro, in vivo, preclinical, investigational, and clinical as well as population large molecule turnover studies. Its main advantage is to determine how product turnover is optimized with disease state and drug effects.

Substrate-product labeling occur via exchange and new synthesis in disease and drug responsive tissue specific large molecule (sterols, peptide, protein; best applied in and above >m/z300) synthesis and turnover.

PRISOMATCH targets common, abundant, non-tracer specific labeled products, while antibody capturing or gel electrophoresis improves specificity. There is no need for annotations or unknown determination during data interpretations.

Precursor-product real time labeling and turnover studies greatly aid clinical decision making for diagnostics, drug dosing and individualized health states in the hand of physician biochemists.

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